C Robin Buell, Michigan State University; Heather L. Merk, The Ohio State University
With the recent release of the potato genome sequence and multiple transcriptome libraries, there is a wealth of data that may be leveraged to improve potatoes. Dr. C. Robin Buell describes the creation of these data and their potential utility. This webinar is divided into four videos.
During the introductory video, Dr. Buell reviews the approaches taken to sequence the potato genome.
During the second video, Dr. Buell describes sequencing of 45 transcriptome libraries using next generation sequencing. These libraries included transcribed sequences (e.g. mRNA) obtained from multiple stages of tuber and plant development, as well as tissue from plants under biotic and abiotic stresses. These transcriptome sequences were used to annotate the potato genome and to infer biological function. Analysis of sequence from the transcriptomes of three varieties (Snowden, Atlantic, and Premier Russet) was used to design the Solanaceae Coordinated Agricultural Project (SolCAP) potato single nucleotide polymorphism (SNP) array.
During the third video, Dr. Buell demonstrates where to obtain the potato genome sequence data and how to view the data using the potato genome browser.
During the fourth video, Dr. Buell demonstrates how to obtain and interpret the potato transcriptome data.
This video contains the full 28 minute recording.
Development of this page was supported in part by the National Institute of Food and Agriculture (NIFA) Solanaceae Coordinated Agricultural Project, agreement 2009-85606-05673, administered by Michigan State University. Any opinions, findings, conclusions, or recommendations expressed in this publication are those of the author(s) and do not necessarily reflect the view of the United States Department of Agriculture.